Farming and earth observation: sentinel-2 data to estimate within-field wheat grain yield

Wheat grain yield (GY) is a crop feature of central importance affecting agricultural, environmental, and socioeconomic sustainability worldwide. Hence, the estimation of within-field variability of GY is pivotal for the agricultural management, especially in the current global change context. In this sense, Earth Observation Systems (EOS) are key technologies that use satellite data to monitor crop yield, which can guide the application of precision farming. Yet, novel research is required to improve the multiplatform integration of data, including data processing, and the application of this discipline in agricultural management. This article provides a novel methodological analysis and assessment of its applications in precision farming. It presents an integration of wheat GY, Global Positioning Systems (GPS), combine harvester data, and EOS Sentinel-2 multispectral bands. Moreover, it compares several indices and machine learning (ML) approaches to map within-field wheat GY. It also analyses the importance of multi-date remote sensing imagery and explores its potential applications in precision agriculture. The study was conducted in Spain, a major European wheat producer. Within-field GY data was obtained from a GPS combine harvester machine for 8 fields over three seasons (2017-2019) and consecutively processed to match Sentinel-2 10 m pixel size. Seven vegetation indices (NDVI, GNDVI, EVI, RVI, TGI, CVI and NGRDI) as well as the biophysical parameter LAI (leaf area index) retrieved with radiative transfer models (RTM) were calculated from Sentinel-2 bands. Sentinel-2 10 m resolution bands alone were also used as variables. Random forest, support vector machine and boosted regressions were used as modelling approaches, and multilinear regression was calculated as baseline. Different combinations of dates of measurement were tested to find the most suitable model feeding data. LAI retrieved from RTM had a slightly improved performance in estimating within-field GY in comparison with vegetation indices or Sentinel-2 bands alone. At validation, the use of multi-date Sentinel-2 data was found to be the most suitable in comparison with single date images. Thus, the model developed with random forest regression (e.g. R-2 = 0.89, and RSME = 0.74 t/ha when using LAI) outperformed support vector machine (R-2 = 0.84 and RSME = 0.92 t/ha), boosting regression (R-2 = 0.85 and RSME = 0.88 t/ha) and multilinear regression (R-2 = 0.69 and RSME = 1.29 t/ha). However, single date images at specific phenological stages (e.g. R-2 = 0.84, and RSME = 0.88 t/ha using random forest at stem elongation) also posed relatively high R-2 and low RMSE, with potential for precision farming management before harvest.A & nbsp;We acknowledge the support of the project PID2019-106650RB-C21 from the Ministerio de Ciencia e Innovacion, Spain. J.S. is a recipient of a FPI doctoral fellowship from the same institution (grant: PRE2020-091907) . J.L.A. acknowledges support from the Institucio Catalana de Recerca i Estudis Avancats (ICREA) , Generalitat de Catalunya, Spain) . S. C.K. is supported by the Ramon y Cajal RYC-2019-027818-I research fellowship from the Ministerio de Ciencia e Innovacion, Spain. We acknowledge the support of Cerealto Siro Group, together with Cristina de Diego and Javier Velasco, technical staff from the company, by providing the wheat yield data. This research was also supported by the COST Action CA17134 SENSECO (Optical synergies for spatiotemporal sensing of scalable ecophysiological traits) funded by COST (European Cooperation in Science and Technology, www.cost.eu)

Effect of ZnO nanoparticles on Zn, Cu, and Pb dissolution in a green bioretention system for urban stormwater remediation.

Stormwater runoff from urban and suburban areas can carry hazardous pollutants directly into aquatic ecosystems. These pollutants, such as metals, nutrients, aromatic hydrocarbons, pesticides, and pharmaceuticals, are very toxic to aquatic organisms. Recently, significant amounts of zinc oxide engineered nanoparticles (ZnO-NPs) have been detected in urban stormwater and its bioretention systems. This raises concerns about a potential increase of stormwater toxicity and reduced performance of the treatment infrastructures. To tackle these issues, we developed a simple, low-cost bioretention system to remediate stormwater and retain ZnO-NPs. This system retained up to 73% Zn, 66% Cu, and >99% Pb. However, the removal efficiency for Pb was lower after adding ZnO-NPs to the system, possibly due to the remobilization of Pb phosphates. The effect of ZnO-NPs on stormwater toxicity and metal accumulation in wetland plants was also evaluated

Zinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc

Stable isotope signature of Zn have shown great promise in elucidating changes in uptake and translocation mechanisms of this metal in plants during environmental changes. Here we tested this potential by investigating the effect of high Zn concentrations on the isotopic fractionation patterns of Phragmites australis (Cav.) Trin. ex Steud. Plants were grown for 40 d in a nutritive solution containing 3.2 µM (sufficient) or 2 mM (toxic) Zn. The Zn isotopic composition of roots, rhizomes, shoots and leaves was analysed. Stems and leaves were sampled at different heights to evaluate the effect of long-distance transport on Zn fractionation. During Zn sufficiency, roots, rhizomes and shoots were isotopically heavy (δ66ZnJMC Lyon = 0.2¿) while the youngest leaves were isotopically light (-0.5 ¿). During Zn excess, roots were still isotopically heavier (δ66Zn = 0.5 ¿) and the rest of the plant was isotopically light (up to -0.5 ¿). The enrichment of heavy isotopes at the roots was attributed to Zn uptake mediated by transporter proteins under Zn sufficient conditions and to chelation and compartmentation in Zn excess. The isotopically lighter Zn in shoots and leaves is consistent with long distance root to shoot transport. The tolerance response of P. australis increased the range of Zn fractionation within the plant and with respect to the environment

The nitrogen contribution of different plant parts to wheat grains: exploring genotype, water, and nitrogen effects

The flag leaf has been traditionally considered as the main contributor to grain nitrogen. However, during the reproductive stage, other organs besides the flag leaf may supply nitrogen to developing grains. Therefore, the contribution of the ear and other organs to the nitrogen supplied to the growing grains remains unclear. It is important to develop phenotypic tools to assess the relative contribution of different plant parts to the N accumulated in the grains of wheat which may helps to develop genotypes that use N more efficiently. We studied the effect of growing conditions (different levels of water and nitrogen in the field) on the nitrogen contribution of the spike and different vegetative organs of the plant to the grains. The natural abundance of 15 δ N and total N content in the flag blade, peduncle, whole spike, glumes and awns were compared to the 15 δ N and total N in mature grains to trace the origin of nitrogen redistribution to the grains. The 15 δ N and total N content of the different plant parts correlated positively with the 15 δ N and total N content of mature grains suggesting that all organs may contribute a portion of their N content to the grains. The potential contribution of the flag blade to grain N increased (by 46%) as the growing conditions improved, whereas the potential contribution of the glumes plus awns and the peduncle increased (46 and 31%, respectively) as water and nitrogen stress increased. In general, potential contribution of the ear providing N to growing grains was similar (42%) than that of the vegetative parts of the plants (30-40%), regardless of the growing conditions. Thus, the potential ear N content could be a positive trait for plant phenotyping, especially under water and nitrogen limiting conditions. In that sense, genotypic variability existed at least between old (tall) and modern (semidwarf) cultivars, with the ear from modern genotypes exhibiting less relative contribution to the total grain N. The combined use of 15 δ N and N content may be used as an affordable tool to assess the relative contribution of different plant parts to the grain N in wheat

Insight. Transgenic solutions to increase yield and stability in wheat: shining hope or flash in the pan?

Second-generation transgenic crops have the potential to transform agriculture, but progress has been limited, and particularly so in wheat where no transgenic cultivar has yet been approved. Taking on the challenge, González et al. (2019) report that transgenic wheat lines carrying a mutated version of the sunflower transcription factor (HaHB4), belonging to the homeodomain-leucine zipper family (HD-Zip I), had increased yield and water use efficiency across a range of environments, with particular benefits under stress. It is an important step forward in an area where progress is urgently needed, though it is too early to claim that transgenic wheat will form the backbone of a second Green Revolution. To meet the growing demand for food, together with the challenges imposed by climate change, substantial improvements in yields of major crops are needed. This includes wheat, where globally the multi-year tendency for growth in yield is decreasing (Passioura, 2012) or even stagnating (Driever et al., 2017). Current and expected future relative rates of progress in yield potential and drought adaptation in wheat are a matter of real concern, and insufficient to meet the projected demand for cereals by 2050 (Hall and Richards, 2013). There are three major challenges: increasing yield potential, protecting yield potential from different types of stress, and increasing resource use efficiency to ensure sustainability (Hawkesford et al., 2013)

Comparison of flag leaf and ear photosynthesis with biomass and grain yield of durum wheat under various water conditions and genotypes

Photosynthetic activity of cereals has traditionally been studied using leaves, thus neglecting the role of other organs such as ears. Here, we studied the effects of water status and genotypes on the photosynthetic activity of the flag leaf blade and the ear of durum wheat. The various parameters related to the photosynthetic activity were analysed in relation to the total above-ground plant biomass and grain yield at maturity. Four local varieties plus two cultivars adapted to the semiarid areas of South Morocco were grown in pots in a greenhouse. Five different water treatments were maintained from the beginning of stem elongation to maturity, when shoot biomass and grain yield were recorded. The net photosynthesis (A), stomatal conductance (gs) and transpiration (T) of the ear and the flag leaf were measured at anthesis. In both organs these factors decreased significantly with water deficit, whereas the A/T and A/gs ratios increased. The genotype effect was also significant for all traits studied. Whole-organ photosynthesis was much higher in the ear than in the flag leaf in well-watered conditions. As water stress developed, photosynthesis decreased less in the ear than in the flag leaf. Whole-ear photosynthesis correlated better than flag leaf photosynthesis with biomass and yield. Nevertheless, the relationships of the whole flag leaf with biomass and yield improved as the water stress became more severe, suggesting a progressive shift of yield from sink to source limitation. For all water regimes the ratios A/gs and A/T of the ear also showed a higher (negative) correlation with both biomass and yield than those of the flag leaf. The results indicate that the ear has a greater photosynthetic role than the flag leaf in determining grain yield, not only in drought but also in the absence of stress

Accumulation and toxic effects of chromium and zinc in Iris pseudacorus L

The aim of the present study was to examine the ability of I. pseudacorus L., an ornamental macrophyte of great potential for phytoremediation, to tolerate and accumulate Cr and Zn. Plants were grown in nutritive solution with ZnCl2 or CrCl3·6H2O at 0, 10, 50, 100, and 200 μg ml−1 for 5 weeks; all survived and continued growing. The accumulation of Cr and Zn increased with increasing supply in all plant tissues, to reach 59.97 mg Cr and 25.64 mg Zn in roots. Leaves retained a remarkable amount of Zn (14.2 mg). Growth inhibition reached 65% and 31% (dry weight) in response to Cr and Zn, respectively. The root:shoot dry matter partitioning (R/S) increased 80% at 100 μg ml−1 CrCl3. The most marked alterations in mineral content were in roots, where both metals decreased Al, Ca, Mg, Mn and S, and increased P concentration. No effect was noted on either leaf chlorophyll fluorescence kinetics (F v /F m and ΦPSII), or photosynthetic pigment content, signifying that the light phase of photosynthesis was not impaired. Carbon isotope composition (δ13C) was only slightly heavier, indicating that the reduction of carbon fixation was not the main cause for growth decrease. This was attributed to the restricted mineral uptake and to the increased demand of carbohydrates of damaged roots. Biomass allocation to rhizomes (Cr) or roots (Zn) contributes to heavy metal tolerance by limiting transpiration and increasing metal-storing tissues and the surface for water and cation uptake. This species is a good candidate for Cr rhizofiltration and Zn phytoextraction